Journal: PLoS Pathogens

Article Title: Immune Antibodies and Helminth Products Drive CXCR2-Dependent Macrophage-Myofibroblast Crosstalk to Promote Intestinal Repair

doi: 10.1371/journal.ppat.1004778

Figure Lengend Snippet: Bone marrow derived MΦ (BMM) or primary small intestinal MF from were co-cultured with Hpb larvae (L3) for 24h with our without immune sera (IS) from challenge Hpb infected mice; (A) CXCL2 in BMM culture supernatants (C57BL/6 or Fcrg -/- ) cultured without (ctr) or with IS, L3 or both; (B) top: CXCL2 staining in control or L3IS-co-cultured BMM, bottom: IgG (red)/ CXCL2 (green) overlay; (C) Scratch wound closure by MF after addition of supernatants from BMM, cultured without (ctr) or with L3 and IS +/- CXCR2 antagonist SB265610. (D) Cxcl2 mRNA induction in MF after IS, L3 or L3 and IS-stimulation relative to unstimulated MF; (E) CXCL2 in culture supernatants from MF, cultured without (ctr) or with L3 or L3 and IS; (F) Overlays of CXCL2 (green) and IgG (red) for unstimulated (control) MF and L3IS-co-cultured C57BL/6 or Fcrg -/- MF; (G) Cxcl2 mRNA induction in MF after L3 co-culture; (H) CXCL2 in MF supernatants after culture without (ctr) or with L3 or L3IS;(I) Cxcl3 mRNA induction in MF after culture in the absence or presence of L3 or L3IS; (J) CXCL3 in unstimulated (control) MF or L3-co-cultured MF; (K) Cxcl3 mRNA induction in MF after L3-co-culture; All data are pooled from at least 2 independent experiments with cells from 3–4 mice per group and presented as mean + SEM.

Article Snippet: Human CXCL3 was quantified using an anti-human CXCL3 ELISA Kit (LuBioScience, Luzern, Switzerland).

Techniques: Derivative Assay, Cell Culture, Infection, Staining, Control, Co-Culture Assay

Journal: PLoS Pathogens

Article Title: Immune Antibodies and Helminth Products Drive CXCR2-Dependent Macrophage-Myofibroblast Crosstalk to Promote Intestinal Repair

doi: 10.1371/journal.ppat.1004778

Figure Lengend Snippet: (A) Human MDM from 3 healthy blood donors (1–3) were co-cultured with A . suum larvae in the absence or presence of IS from challenge- A . suum infected pigs and time-lapse movies were recorded (2 frames/ s, 1 min); upper panel: representative snap shots at T = 0; lower panel: representative temporal color code images (120 frames, 1 min)—colors represent different time points; Scale bars 100 μm; (B) Adherent MDM per A . suum larva were counted in time-lapse movies; (C) CXCL3 in culture supernatants from human MDM cultured with or without immune sera from Ascaris -infected pigs (IS), Ascaris suum larvae ( A . s .) or both ( A . s . IS) quantified by ELISA; (D) Scratch wound closure by human MF cultured in the presence of conditioned media from MDM (MDM sup) stimulated with A . s . IS +/- the CXCR2 antagonist SB265610; All data are pooled from at least 3 independent experiments with n = 3 co-cultures in each and presented as mean +/-SEM.

Article Snippet: Human CXCL3 was quantified using an anti-human CXCL3 ELISA Kit (LuBioScience, Luzern, Switzerland).

Techniques: Cell Culture, Infection, Enzyme-linked Immunosorbent Assay